CRISPR genome surgery in a novel humanized model for autosomal dominant retinitis pigmentosa

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High-performance multiplex drug-gated CAR circuits

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江西省县（区）级疾控中心职业卫生技术能力状况调查分析

[摘要]?目前研究已知HBV有多种基因型，不同基因型在不同的种族、地域方面分布特点不同且HBV基因型是造成慢性感染自然史差异的原因之一，它们在感染的临床表现和抗病毒治疗的反应中起着重要作用。检测HBV基因型和了解HBV不同基因型对疾病预后的影响，对评估患者疾病结局和指导临床规划最佳治疗方案具有重要意义。 　　[关键词]　乙型肝炎病毒；基因型；基因突变；治疗     

Comparison of dystrophin expression following gene editing and gene replacement in an aged preclinical DMD animal model

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Local magnetic delivery of adeno-associated virus AAV2(quad Y-F)-mediated BDNF gene therapy restores hearing after noise injury

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层流洁净新生儿重症监护病房早产儿实施床旁手术的可行性分析

 目的 探讨层流洁净新生儿重症监护病房(NICU)早产儿实施床旁手术的效果与可行性。方法 回顾性分析某军队三甲综合医院2017年9月—2020年10月NICU住院并进行床旁动脉导管扎闭术(PDA)及腹部探查术的危重新生儿的临床资料,在NICU行床旁手术者列为NICU组,转入手术室进行手术者列为手术室(OR)组,比较两组新生儿术后体温、切口感染率及病死率的差异。结果 共计纳入258例新生儿,其中NICU组166例,OR组92例。NICU组新生儿出生胎龄、出生体质量、手术时体质量、新生儿危重病例评分均低于OR组;NICU组新生儿术前应用呼吸机通气比例高于OR组,差异均有统计学意义(均P＜0.05)。NICU组与OR组新生儿术中抽检空气培养微生物菌落数、术前体温、PDA与剖腹探查术的构成比例、术后切口感染发病率及病死率比较,差异均无统计学意义(均P＞0.05),但手术后OR组新生儿平均体温低于NICU组,差异有统计学意义(P＜0.05)。结论 层流洁净NICU早产儿行床旁PDA和腹部手术临床可行,且床旁手术更有利于减少术后低体温的发生。对层流洁净病房进行日常规范化维护,可以有效保障危重早产儿床旁手术的安全。     

The effect of various in-vitro and ex-vivo parameters on irrigant flow and apical pressure using manual syringe needle irrigation: Systematic review

BackgroundFluid dynamics is a majorly neglected aspect to be studied in root canal irrigation. The fundamental rule to understand mechanics is to observe patterns of flow during the process. Thus, this work is conducted to do a systemic assessment of the in-vitro and ex-vivo based studies to evaluate the effect of various parameters on the irrigant flow and apical pressure on using a manual syringe needle for root canal irrigation.MethodsThe literature search was conducted through libraries such as PubMed (Medline), CINAHL, Embase, Scopus and other hand literature from Google Scholar, the British medical library etc. The systematic review was reported following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. As they include studies that were in-vitro and ex-vivo based, the risk of bias of the selected articles was assessed using a customized tool based on the previous literature and parameters evaluated in the studies included.ResultsThe literature search resulted in 101 items of which 19 records were included in this review. Results reported that multiple factors and parameters were assessed to evaluate the flow and apical pressures on using manual syringe needle irrigation.ConclusionsPresent systematic review gives insights in-depth about the irrigation dynamics of manual syringe needle irrigation. Besides, it is inconclusive to compile a single factor or a single parameter contributing to the enhanced irrigant flow and least apical pressures.     

Correlation analysis of intestinal flora and pathological process of type 2 diabetes mellitus

ObjectiveTo observe the relationship between the different stages of type 2 diabetes mellitus (T2DM) and the intestinal flora and verify its underlying mechanism.MethodsT2DM rats were generated by high-fat diet (HFD) combined with intraperitoneal streptozotocin (STZ) injection. The rats were divided into four groups: the control group (fed with normal feed for 1 month), the HFD group (fed with HFD for 1 month), the T2DM group (HFD combined with STZ and blood glucose ≥11.1 mM), and the unformed T2DM model (Un-mod) group (HFD combined with STZ and blood glucose <11.1 mM). Feces were collected, and bacterial communities in the fecal samples were analyzed by 16S rRNA gene sequencing. The content of short-chain fatty acids (SCFAs) in feces was measured by gas chromatography. Western blot and quantitative real-time polymerase chain reaction were used to detect the expression of G protein-coupled receptor 41 (GPR41) and GPR43.ResultsAt different stages of T2DM, the intestinal flora and SCFAs content of rats were significantly decreased (all P < .05). Our results indicated that g__Prevotella had a significant negative correlation, and g__Ruminococcus_torques_group and g__lachnoclastic had a significant positive correlation with blood glucose. The content of SCFAs, in particular acetate and butyrate, in rat feces of different stages of T2DM were significantly reduced, as well as GPR41 and GPR43 expression. The results in the Un-mod group were similar to the T2DM group, and the expression of GPR41 and GPR43 proteins were significantly higher than those in the T2DM group (both P < .001).ConclusionThe intestinal flora–SCFAs–GPR41/GPR43 network may be important in the development of T2DM. Decreasing blood glucose levels by regulating the intestinal flora may become a new therapeutic strategy for T2DM, which has very important clinical and social values.